BTTAA 是最新一代的 CuAAC 水溶性加速配體，與上一代配體（例如 THPTA 或 TBTA）相比，它提供了更大的速率增強。更重要的是，它最大限度地減少了對所探測細胞或生物體生理狀態的擾動，并通過進一步降低催化劑配方中的銅負載量，實現了有效的生物偶聯，抑制了細胞毒性。

BTTAA is a newest generation, water-soluble accelerating ligand for CuAAC that provides much greater rate enhancement compared to previous generation ligands (e.g. THPTA or TBTA). More importantly, it minimizes perturbations to the physiological state of the cells or organisms probed and allows for effective bioconjugation with suppressed cell cytotoxicity by further lowering copper loading in the catalyst formulation.